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scintillation proximity assay spa beads  (Revvity)


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    Structured Review

    Revvity scintillation proximity assay spa beads
    Scintillation Proximity Assay Spa Beads, supplied by Revvity, used in various techniques. Bioz Stars score: 90/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/scintillation+proximity+assay+spa+beads/WGA+PVT+500+MG+SPA+Beads/pm33939425-707-29-53
    Average 90 stars, based on 16 article reviews
    scintillation proximity assay spa beads - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Serial Dilution:

    Article Title: Discovery of the S1P2 Antagonist GLPG2938 (1-[2-Ethoxy-6-(trifluoromethyl)-4-pyridyl]-3-[[5-methyl-6-[1-methyl-3-(trifluoromethyl)pyrazol-4-yl]pyridazin-3-yl]methyl]urea), a Preclinical Candidate for the Treatment of Idiopathic Pulmonary Fibrosis.
    Article Snippet: Mounting evidence from the literature suggests that blocking S1P2 receptor (S1PR2) signaling could be effective for the treatment of idiopathic pulmonary fibrosis (IPF).. However, only a few antagonists have been so far disclosed.. A chemical enablement strategy led to the discovery of a pyridine series with good antagonist activity.

    Membrane:

    Article Title: Discovery of the S1P2 Antagonist GLPG2938 (1-[2-Ethoxy-6-(trifluoromethyl)-4-pyridyl]-3-[[5-methyl-6-[1-methyl-3-(trifluoromethyl)pyrazol-4-yl]pyridazin-3-yl]methyl]urea), a Preclinical Candidate for the Treatment of Idiopathic Pulmonary Fibrosis.
    Article Snippet: Mounting evidence from the literature suggests that blocking S1P2 receptor (S1PR2) signaling could be effective for the treatment of idiopathic pulmonary fibrosis (IPF).. However, only a few antagonists have been so far disclosed.. A chemical enablement strategy led to the discovery of a pyridine series with good antagonist activity.

    Scintillation Proximity Assay:

    Article Title: Discovery of the S1P2 Antagonist GLPG2938 (1-[2-Ethoxy-6-(trifluoromethyl)-4-pyridyl]-3-[[5-methyl-6-[1-methyl-3-(trifluoromethyl)pyrazol-4-yl]pyridazin-3-yl]methyl]urea), a Preclinical Candidate for the Treatment of Idiopathic Pulmonary Fibrosis.
    Article Snippet: Mounting evidence from the literature suggests that blocking S1P2 receptor (S1PR2) signaling could be effective for the treatment of idiopathic pulmonary fibrosis (IPF).. However, only a few antagonists have been so far disclosed.. A chemical enablement strategy led to the discovery of a pyridine series with good antagonist activity.

    Article Title: Discovery of 9-Cyclopropylethynyl-2-((S)-1-[1,4]dioxan-2-ylmethoxy)-6,7-dihydropyrimido[6,1-a]isoquinolin-4-one (GLPG1205), a Unique GPR84 Negative Allosteric Modulator Undergoing Evaluation in a Phase II Clinical Trial
    Article Snippet: .. First, 50 μL of hit compound from HTS was added into the assay plate, 747 followed by addition of 20 μL DIM at EC80 concentration (concentration which give 748 80% of the activity of GPR84) and were incubated with 30 μL of mixture consisting of 749 membranes derived from stable cell line overexpressing recombinant human 750 GPR84, [35S]GTPγS and scintillation proximity assay (SPA) beads (Perkin Elmer, 751 Boston, MA, USA; RPNQ0001). ..

    Derivative Assay:

    Article Title: Discovery of the S1P2 Antagonist GLPG2938 (1-[2-Ethoxy-6-(trifluoromethyl)-4-pyridyl]-3-[[5-methyl-6-[1-methyl-3-(trifluoromethyl)pyrazol-4-yl]pyridazin-3-yl]methyl]urea), a Preclinical Candidate for the Treatment of Idiopathic Pulmonary Fibrosis.
    Article Snippet: Mounting evidence from the literature suggests that blocking S1P2 receptor (S1PR2) signaling could be effective for the treatment of idiopathic pulmonary fibrosis (IPF).. However, only a few antagonists have been so far disclosed.. A chemical enablement strategy led to the discovery of a pyridine series with good antagonist activity.

    Article Title: Discovery of 9-Cyclopropylethynyl-2-((S)-1-[1,4]dioxan-2-ylmethoxy)-6,7-dihydropyrimido[6,1-a]isoquinolin-4-one (GLPG1205), a Unique GPR84 Negative Allosteric Modulator Undergoing Evaluation in a Phase II Clinical Trial
    Article Snippet: .. First, 50 μL of hit compound from HTS was added into the assay plate, 747 followed by addition of 20 μL DIM at EC80 concentration (concentration which give 748 80% of the activity of GPR84) and were incubated with 30 μL of mixture consisting of 749 membranes derived from stable cell line overexpressing recombinant human 750 GPR84, [35S]GTPγS and scintillation proximity assay (SPA) beads (Perkin Elmer, 751 Boston, MA, USA; RPNQ0001). ..

    Stable Transfection:

    Article Title: Discovery of the S1P2 Antagonist GLPG2938 (1-[2-Ethoxy-6-(trifluoromethyl)-4-pyridyl]-3-[[5-methyl-6-[1-methyl-3-(trifluoromethyl)pyrazol-4-yl]pyridazin-3-yl]methyl]urea), a Preclinical Candidate for the Treatment of Idiopathic Pulmonary Fibrosis.
    Article Snippet: Mounting evidence from the literature suggests that blocking S1P2 receptor (S1PR2) signaling could be effective for the treatment of idiopathic pulmonary fibrosis (IPF).. However, only a few antagonists have been so far disclosed.. A chemical enablement strategy led to the discovery of a pyridine series with good antagonist activity.

    Article Title: Discovery of 9-Cyclopropylethynyl-2-((S)-1-[1,4]dioxan-2-ylmethoxy)-6,7-dihydropyrimido[6,1-a]isoquinolin-4-one (GLPG1205), a Unique GPR84 Negative Allosteric Modulator Undergoing Evaluation in a Phase II Clinical Trial
    Article Snippet: .. First, 50 μL of hit compound from HTS was added into the assay plate, 747 followed by addition of 20 μL DIM at EC80 concentration (concentration which give 748 80% of the activity of GPR84) and were incubated with 30 μL of mixture consisting of 749 membranes derived from stable cell line overexpressing recombinant human 750 GPR84, [35S]GTPγS and scintillation proximity assay (SPA) beads (Perkin Elmer, 751 Boston, MA, USA; RPNQ0001). ..

    Concentration Assay:

    Article Title: Discovery of 9-Cyclopropylethynyl-2-((S)-1-[1,4]dioxan-2-ylmethoxy)-6,7-dihydropyrimido[6,1-a]isoquinolin-4-one (GLPG1205), a Unique GPR84 Negative Allosteric Modulator Undergoing Evaluation in a Phase II Clinical Trial
    Article Snippet: .. First, 50 μL of hit compound from HTS was added into the assay plate, 747 followed by addition of 20 μL DIM at EC80 concentration (concentration which give 748 80% of the activity of GPR84) and were incubated with 30 μL of mixture consisting of 749 membranes derived from stable cell line overexpressing recombinant human 750 GPR84, [35S]GTPγS and scintillation proximity assay (SPA) beads (Perkin Elmer, 751 Boston, MA, USA; RPNQ0001). ..

    Activity Assay:

    Article Title: Discovery of 9-Cyclopropylethynyl-2-((S)-1-[1,4]dioxan-2-ylmethoxy)-6,7-dihydropyrimido[6,1-a]isoquinolin-4-one (GLPG1205), a Unique GPR84 Negative Allosteric Modulator Undergoing Evaluation in a Phase II Clinical Trial
    Article Snippet: .. First, 50 μL of hit compound from HTS was added into the assay plate, 747 followed by addition of 20 μL DIM at EC80 concentration (concentration which give 748 80% of the activity of GPR84) and were incubated with 30 μL of mixture consisting of 749 membranes derived from stable cell line overexpressing recombinant human 750 GPR84, [35S]GTPγS and scintillation proximity assay (SPA) beads (Perkin Elmer, 751 Boston, MA, USA; RPNQ0001). ..

    Incubation:

    Article Title: Discovery of 9-Cyclopropylethynyl-2-((S)-1-[1,4]dioxan-2-ylmethoxy)-6,7-dihydropyrimido[6,1-a]isoquinolin-4-one (GLPG1205), a Unique GPR84 Negative Allosteric Modulator Undergoing Evaluation in a Phase II Clinical Trial
    Article Snippet: .. First, 50 μL of hit compound from HTS was added into the assay plate, 747 followed by addition of 20 μL DIM at EC80 concentration (concentration which give 748 80% of the activity of GPR84) and were incubated with 30 μL of mixture consisting of 749 membranes derived from stable cell line overexpressing recombinant human 750 GPR84, [35S]GTPγS and scintillation proximity assay (SPA) beads (Perkin Elmer, 751 Boston, MA, USA; RPNQ0001). ..

    Recombinant:

    Article Title: Discovery of 9-Cyclopropylethynyl-2-((S)-1-[1,4]dioxan-2-ylmethoxy)-6,7-dihydropyrimido[6,1-a]isoquinolin-4-one (GLPG1205), a Unique GPR84 Negative Allosteric Modulator Undergoing Evaluation in a Phase II Clinical Trial
    Article Snippet: .. First, 50 μL of hit compound from HTS was added into the assay plate, 747 followed by addition of 20 μL DIM at EC80 concentration (concentration which give 748 80% of the activity of GPR84) and were incubated with 30 μL of mixture consisting of 749 membranes derived from stable cell line overexpressing recombinant human 750 GPR84, [35S]GTPγS and scintillation proximity assay (SPA) beads (Perkin Elmer, 751 Boston, MA, USA; RPNQ0001). ..



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